The Role of Eruca Sativa Extract on the Toxic Effect of Proton Pump Inhibitor on Cerebellar Cortex in Adult Males Albino Rat

Abstract

Objectives: Proton pump inhibitors produce marked histopathological changes in the cerebellar cortex that it could disturb the normal function of cerebellum. The aim of this work is to study, for the first time, the role of eruca sativa in the protection and treatment of these neuropathological changes that could be detected in the cerebellar cortex by using proton pump inhibitor. Material and Methods: Sixty adult males albino rat were used and divided into five main groups; Group I: contained twenty animals and was further subdivided into two subgroups, Group II: (Eruca treated group) included ten animals, Group III: (Proton pump inhibitor group) included ten animals that received treatment of pantoprazole in an oral dose of 1.3 mg /kg dissolved in 1ml phosphate–buffered saline by gastric tube once daily for 4 weeks. Group IV: (Protected group) included ten animals received Eruca sativa seeds extract daily for 4 weeks and pantoprazole daily for 4 weeks started from 2nd day of the experiment. Group V (Treated group): included ten animals received pantoprazole once daily for 4 weeks followed by a daily oral dose of Eruca sativa seeds extract for 4 weeks.Results: Regardingto the histological, immunohistochemical and morphometric studies; eruca sativa significantly protected the cerebellar cortex from the damaging effects of pantoprazole and significant improvement was also observed when it was used as a treatment. Conclusion: Eruca sativa played an important role in protection and treatment of the cerebellar cortex against the neuropathological changes that could be detected by using the proton pump inhibitor; pantoprazole.