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Abstract
The role of donor-acceptor interactions in base pair stacking in DNA and RNA has been minimized because of the perceived low or negative electron affinities of the purines and pyrimidines. The use of the electron capture detector was among the first methods for measuring electron affinities in the gas phase. Recently, the experimental determination of electron affinities has been extended and improved. Now, there are data for similar compounds in the literature which enable us to estimate electron affinities for purines and pyrimidines. These values are significant, and positive, such that donor-acceptor interactions can, and indeed should play a role in the stacking of bases in nucleic acids.
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