Abstract
Granulocyte colony-stimulating factor (G-CSF) exerts various biological effects through occupancy of its receptor (G-CSFR). WEHI-3B D - myelomonocytic leukemia cells do not express the G-CSFR, do not respond to G-CSF or to retinoic acid by the induction of granulocytic maturation, contain a near tetraploid content of DNA, and form tightly aggregated colonies. However, they still maintain the capacity to differentiate since they respond to vitamin D 3 by the formation of mature cells. Transfection of the G-CSFR gene into WEHI-3B D - cells resulted in three major changes. G-CSFR-expressing clones (a) acquired the capacity to respond to the differentiation-inducing properties of G-CSF and retinoic acid, (b) formed colonies which exhibited a dispersed phenotype, and (c) exhibited near diploid DNA ploidy. In contrast, WEHI-3B D - cells transfected with vector alone behaved like parental WEHI-3B D - cells. The findings imply that the near diploid phenotype is required for WEHI-3B D - leukemia cells to respond to certain inducers of differentiation.
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