The role of differentially expressed miR-660 in peripheral blood lymphocytes of patients with pulmonary tuberculosis

Abstract

Objective: This study aimed to investigate the significance of miRNA expression levels in peripheral blood lymphocytes of patients clinically diagnosed with pulmonary tuberculosis. Method: Pulmonary tuberculosis-related datasets in the Gene Expression Omnibus (GEO) database were analyzed, and DE-miRNAs were screened for Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment to construct a DE-miRNA-DE-mRNA network. The peripheral blood lymphocytes of 10 patients with pulmonary tuberculosis, 10 patients with rifampicin-resistant tuberculosis, and 10 healthy volunteers were selected for validation of RNA expression levels. qRT-PCR was done to verify the expression of DE-miRNA, and Western blotting was done to check the expression levels of genes of associated pathways. Results: Differential expression of miR-660 was found in pulmonary tuberculosis through data analysis and literature mining. The differential expression was also confirmed by qRT-PCR in samples from patients and healthy controls. The expression of miR-660 was significantly upregulated (p < 0.01) in pulmonary tuberculosis and rifampicin-resistant pulmonary tuberculosis patients compared to the healthy controls. According to western blotting results, the expression levels of P-NF-κB and AKT in pulmonary tuberculosis patients and NF-κB, P-NF-κB, AKT, and p-AKT in rifampicin-resistant tuberculosis patients were significantly upregulated (p < 0.01). Conclusion: The high expression levels of miR-660 may activate the AKT/NF-κB signaling pathway and has the potential to serve as a potential biomarker for the diagnosis of pulmonary tuberculosis.