Abstract
AimBrucellar spondylitis (BS) is one of the most serious complications of brucellosis. CXCR3 is closely related to the severity of disease infection. This research aimed to study the degree of BS inflammatory damage through analyzing the expression levels of CXCR3 and its ligands (CXCL9 and CXCL10) in patients with BS.MethodsA total of 29 BS patients and 15 healthy controls were enrolled. Real-Time PCR was used to detect the mRNA expression levels of IFN-γ, CXCR3, CXCL9 and CXCL10 in peripheral blood mononuclear cells (PBMCs) of BS patients and healthy controls. Hematoxylin-Eosin staining was used to show the pathological changes in BS lesion tissues. Immunohistochemistry staining was used to show the protein expression levels of Brucella-Ab, IFN-γ, CXCR3, CXCL9 and CXCL10 in BS lesion tissues. At the same time, ELISA was used to detect the serum levels of IFN-γ, CXCL9 CXCL10 and autoantibodies against CXCR3 in patients with BS.ResultsIn lesion tissue of BS patients, it showed necrosis of cartilage, acute or chronic inflammatory infiltration. Brucella-Ab protein was abundantly expressed in close lesion tissue. And the protein expression levels of IFN-γ, CXCR3 and CXCL10 were highly expressed in close lesion tissue and serum of BS patients. At the same time, the mRNA expression levels of IFN-γ, CXCR3 and CXCL10 in PBMCs of BS patients were significantly higher than those in controls.ConclusionIn our research, the expression levels of IFN-γ, CXCR3 and its ligands were significantly higher than those in controls. It suggested that high expression levels of IFN-γ, CXCR3 and its ligands indicated a serious inflammatory damage in patients with BS.
Highlights
Brucella is a facultative anaerobic intracellular bacterium that causes brucellosis in human
We demonstrated that the CXCR3 and its ligands (CXCL9 and CXCL10) are highly expressed in Brucella infected tissues and peripheral blood mononuclear cells (PBMCs)
This study focus on analyzing the expression levels of CXCR3 and its IFN-γ-induced ligands (CXCL9 and CXCL10) in Brucellar spondylitis (BS) patient tissues and PBMCs
Summary
Brucella is a facultative anaerobic intracellular bacterium that causes brucellosis in human. Brucellosis is a contagious zoonotic disease, known as undulant fever. The Brucella infects human through direct or indirect contact with contaminated animal products (such as eating raw meat or unpasteurized dairy products) [1]. It is mainly distributed in Central America, South America, Africa and Asia [2]. It was listed as a class B infectious disease in China. The high morbidity percent of brucellosis is an average of 36.01 / 100,000 from 2014 to 2016 [3]
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