The role of Coenzyme Q10on the Total Antioxidant Capacity of Mouse Vitrified Pre-Antral Follicles

Abstract

Occurrenceofoxidative stress (OS) following in vitro culture is inescapable. Therefore, the aim of the present study was to determine the efficacy of Coenzyme Q 10 (CoQ 10 ) supplementation medium on developments and Total Antioxidant Capacity (TAC) of mouse vitrified pre-antral follicles. Fresh and vitrified-warmed isolated preantral follicles from ovaries of 14-16 day s -old mice were cultured in supplemented α- MEM medium with or without CoQ 10 .On the twelfth day of culture period,ovulation was induced by adding 1.5 IU/ml human Chorionic Gonadotropin (hCG). Rates of survival, antrum formation and developmental stages of released oocytes (GV, MI, MII) were evaluated. Separately,total antioxidant capacity (TAC) levels were measured at initial time, 24, 48, 72 and 96 hours of culture periodby ferric reducing/antioxidant power (FRAP) assay. The results showed that the mean diameter and rates of survival, antrum formation, ovulation and MII oocytes of fresh and vitrified-warmed isolated preantral follicles with pretreatment of CoQ 10 were significantly higher than those of respective groups without pretreatment ofCoQ 10 . TAC levels significantly increased up to 96hours in fresh and vitrified-warmed preantral follicles with pre-treatment of CoQ 10 compared with thosewhich were cultured without CoQ 10 . Supplemented culture medium with CoQ 10 promotes TAC levels and development of both fresh and vitrified-warmed isolated preantral follicles.