Abstract
The effect of carbon dioxide concentration on the growth of Thalassiosira pseudonana (Hustedt) Hasle and Heimdal clone 3H was studied at three light intensity levels and two oxygen concentrations. Algal cultures were grown in an enriched artificial seawater medium under constant illumination in 500 ml gas wash bottles. Population density, particulate carbon and particulate nitrogen were measured periodically during exponential growth. Growth rate did not increase as the CO 2 concentration was elevated above 16.0 μmole/l. Freshly inoculated algal cultures did not grow in an organic carbon-free medium bubbled with CO 2 -free air. The kinetics of inorganic carbon mobilization including concentrations, reaction rates, and CO 2 transport, generation and uptake are analyzed.
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