Abstract
The Role of Citrate on the Respiratory Control of Isolated Rat Heart Sarcosomes
Highlights
Rat heart sarcosomes were prepared according to the method described for brain mitochondria by Voss, Campello, and Bacila (11)) using a mannitol-sucrose medium
Due to the fact that in a medium containing citrate, heart sarcosomes show respiratory control, experiments were carried out to determine the effect of citrate on the aging of mitochondria (Fig. 1)
The uncoupling of oxidative phosphorylation shown for skeletal muscle mitochondria by Chappell and Perry (17), and for brain and cerebellum by Bacila et al (18) has been attributed to the mitochondrial ATPase
Summary
Rat heart sarcosomes were prepared according to the method described for brain mitochondria by Voss, Campello, and Bacila (11)) using a mannitol-sucrose medium. Three different preparations were carried out: (a) one with an extraction medium containing 0.21 M mannitol, 0.075 M sucrose, and 0.01 M Tris,. T Permanent address, Departamento de Farmacologia, Faculdade de Medicina, Universidade de Minas Gerais, Belo Horisonte, Minas Gerais, Brasil. Rat heart was finely minced and homogenized in a Potter-Elvehjem (12) homogenizer with one of the above mentioned media. The whole suspension was centrifuged 10 minutes at 1,000 x g, the sedimented fraction discarded and the supernatant centrifuged 10 minutes at 10,000 x g. The last sedimented fraction was washed with the extraction medium and centrifuged twice. The pellet was resuspended in the same extraction medium
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