The Role of CDR1as in Proliferation and Differentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells.

Lunyu Yang,Xinye Han,Peipei Wu,Wenrong Xu,Hui Qian,Li Rong,Benshuai You,Zhang Bin,Shi Hui

doi:10.1155/2019/2316834

Abstract

Mesenchymal stem cells derived from human umbilical cord (hucMSCs) are considered a promising tool for regenerative medicine. circRNAs as newly discovered noncoding RNAs are involved in multiple biological processes. However, little has been known about the function of circRNAs in the proliferation and differentiation of hucMSCs. In this study, we selected several circRNAs expressed in MSCs from circBase and found that CDR1as expression level was markedly significant. We observed that, compared with that of uninduced hucMSCs, the CDR1as expression level of induced hucMSCs decreased with cell induction differentiation. By using siRNA to knock down CDR1as of hucMSCs, we discovered that proliferation was inhibited but the apoptosis increased. In addition, we found that the expression of stemness transcription factors (STFs) was downregulated after CDR1as knockdown and the adipogenesis and osteogenesis potential of hucMSCs was impaired. Our findings suggest that CDR1as takes a part in maintaining proliferation and differentiation of hucMSCs, providing clues for MSC modification and further for stem cell therapy and tissue regeneration.

Highlights

Mesenchymal stem cells (MSCs) are a group of multipotent stem cells with characteristics of self-renewal and multipotency to differentiate into adipocytes, osteoblasts, chondrocytes, and other lineage cells [1]
To further investigate the expression level of CDR1as in other cells, we collected RNAs from gastric cancer cells, gastric epithelial cells, MSCs isolated from gastric cancer tissue and human umbilical cord, and 293 T cells
Compared with cancer cells or epithelial cell, CDR1as was highly expressed in MSCs with significance, and we found that CDR1as expression level in 293 T cells was similar to that in MSCs (Figure 1(b))

Summary

Introduction

Mesenchymal stem cells (MSCs) are a group of multipotent stem cells with characteristics of self-renewal and multipotency to differentiate into adipocytes, osteoblasts, chondrocytes, and other lineage cells [1]. MSCs have been isolated from various tissues including bone morrow, endodontic pulp, umbilical cord, hair follicle, adipose, and other tissues [2,3,4,5,6]. Due to their proliferation and differentiation potential as well as the established protocols for their isolation and propagation in vitro, MSCs have become attractive tools for tissue injury repair and regenerative medicine [7]. Engrafted MSCs could differentiate into tissue-specific cells or function-related cells required for tissue regeneration [8, 9]. In vitro and in vivo studies have indicated that MSCs played a powerful role in tissue injury repair, the molecular mechanism that regulates the proliferation and differentiation of MSCs has not yet been clarified

Methods

Results

Conclusion