The Role of Cavin3 in the Progression of Lung Cancer and Its Mechanism.

Gaozhong Sun,Kewei Ni

doi:10.1155/2020/6364801

Abstract

Objective The purpose of this study was to describe the role of Cavin3 in the progression of lung cancer and its underlying mechanism. Methods Totally, 200 cases of lung cancer tissues and corresponding paracancer tissues were collected. Cavin3 expression in samples was determined by qRT-PCR, and the correlation with lung cancer stages as well as prognosis was statistically analyzed combined with matched clinical information. To investigate the mechanism of Cavin3 in lung cancer progression, firstly, Cavin3 was detected in lung cancer cell lines A549, PC9, and H520. Then, cells with stable Cavin3 overexpression and Cavin3 knockout were established to determine the effect of Cavin3 overexpression on the mammalian target of rapamycin (mTOR) signaling pathway. Subsequently, cells were harvested for cell proliferation, migration, and invasion assays in vitro, as well as nude mouse transplantation tumor experiment in vivo. Results Cavin3 was seen to be highly expressed in cancer tissues. Statistical analysis with matched clinical data showed that Cavin3 as a prognostic indicator of lung cancer had important clinical value. In addition, it could be found that high expression of Cavin3 was able to promote cell proliferation, migration, and invasion and also potentiate tumor formation in vivo. Conclusion Cavin3 was highly expressed in lung cancer, and it was capable to promote cell proliferation, invasion, and migration.

Highlights

Lung cancer is one of the most serious malignancies threatening people’s health and life, and it has become a public health issue of great concern throughout the world [1]
A variety of cellular signaling pathways were shown to be involved in the lung cancer progression, among which the mammalian target of rapamycin signal exhibits a vital role in many other tumors as proved by a large amount of evidence [5]
Studies have reported that the protein level and functional role of Cavin in cancer cells are closely related to cancer metabolism and stress signaling [10, 11]

Summary

Introduction

Lung cancer is one of the most serious malignancies threatening people’s health and life, and it has become a public health issue of great concern throughout the world [1]. For better efficacy and prognosis, it is crucial to clarify the molecular mechanism as well as explore effective biomarkers in lung cancer tumorigenesis and development. A variety of cellular signaling pathways were shown to be involved in the lung cancer progression, among which the mammalian target of rapamycin (mTOR) signal exhibits a vital role in many other tumors as proved by a large amount of evidence [5]. In our previous study on signal molecule DEPTOR (endogenous mTOR inhibitor) in lung cancer, we found that Cavin was an important protein involved in the mTOR signaling pathway. The effect of Cavin on tumorigenesis and development has not been further studied, which enables us to make more efforts on the underlying mechanism and to clarify the relationship between Cavin and mTOR signaling pathway

Methods

Results

Conclusion