Abstract
Carbon source plays an important role in the induction of embryogenic callus (EC). Therefore it is necessary to determine the effective carbohydrate for improving this process. In this study, different carbohydrates as sucrose, maltose, and sorbitol with different levels (0–30 g/L) were tested in the callus induction of Pyrus communis. At first, in solid culture, calli were divided in relation to their morphological appearance in EC and non-EC. EC was white or yellow with the granular aspect. The morphological study revealed the highest frequency of EC (28.5%) obtained at 30 g/L sucrose. Biochemical analyses showed EC exhibited both soluble sugar and protein high contents, while the starch was at the lowest level. As well as flow cytometry showed EC were genetically similar to donor explants. With transferring the EC into the liquid culture, then, subculturing calli along with 2 ml cell suspension on solid medium led to the globular embryo.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
