Abstract
The clinical use of bioactive material in the field of biomedical tissue engineering has become increasingly of interest in practice. This study investigates how BiodentineTM (BD), a tricalcium silicate cement, in culture media, affects the odonto/osteogenic differentiation potential of in vitro cultured human dental pulp stem cells (hDPSCs). hDPSCs were extracted and characterized for their expression profile by flow cytometry. Then, hDPSCs were cultured in media containing BD for 3 weeks to study the impact of BD on the odonto/osteogenesis pathway, compared to the positive control (osteogenic media) and negative control (cell culture media). Odonto/osteogenic differentiation of hDPSCs treated with BD was assessed by measuring the level of expression of odonto/osteogenic markers by flow cytometry, ELISA and Alizarin red stain. Additionally, the expression profile of the genes involved in the odonto/osteogenesis pathway was investigated, using PCR array. Our results indicate that hDPSCs treatment with BD results in an increased tendency for odonto/osteogenic differentiation. The BD treated group demonstrates a significant increase in the expression of odonto/osteogenic markers, osteocalcin (OCN) (p < 0.005), osteopontin (OPN) (p < 0.0005) and alkaline phosphatase (ALP) (p < 0.0005), and the presentation of calcium deposits by ARS, compared to the negative control by using t-test and ANOVA. Moreover, the BD-treated group is marked by the upregulation of genes related to the odonto/osteogenesis pathway, compared to the control groups, specifically the genes that are involved in the bone morphogenic protein (BMP) (p < 0.05) signaling pathway, the activation of the extracellular matrix-related gene (ECMG) (p < 0.05) and the Ca2+ signaling pathway (p < 0.05), compared to day 1 of treatment by using ANOVA. BD shows a stimulatory effect on the odonto/steogenic capacity of hDPSCs, suggesting BD as a good candidate and a very promising and useful means to be applied in regenerative medicine to regenerate dentine tissue in clinical settings.
Highlights
IntroductionMesenchymal stem cells (MSCs) of dental origin have been isolated from different dental tissues, such as (SHED) stem cells from human exfoliated deciduous teeth [4], (PDLSCs) periodontal ligament stem cells [5], (SCAP) stem cells of apical papilla tissue of impacted third molars [6], (DFSCs) dental follicle stem cells [7] and stem cells from periapical cysts [8]
Since no previous studies have evaluated these networks, our study aims to investigate the odonto/steogenic potential of human dental pulp stem cells (hDPSCs) combined with the bioactive material BiodentineTM
The derived hDPSCs were characterized for their mesenchymal stem cells (MSCs) expression profile
Summary
Mesenchymal stem cells (MSCs) of dental origin have been isolated from different dental tissues, such as (SHED) stem cells from human exfoliated deciduous teeth [4], (PDLSCs) periodontal ligament stem cells [5], (SCAP) stem cells of apical papilla tissue of impacted third molars [6], (DFSCs) dental follicle stem cells [7] and stem cells from periapical cysts [8] All these cells are derived from the same dental origin, but they vary in terms of MSC surface markers, and in their differentiation potential for different cell-lineages [9]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
