The role of bacteriophage T7 exonuclease (gene 6) in genetic recombination and production of concatemers

Abstract

Genetic analysis reported here shows that bacteriophage T7 exonuclease (gene 6) is necessary for intragenic and intergenic recombination in several areas of the T7 genetic map. This supports our previous conclusion ( Lee & Miller, 1974) that the enzyme is necessary for T7 molecular recombination. Results of sucrose gradient analysis show that DNA concatemers are formed when both the T7 exonuclease (gene 6) and the T7 endonuclease (gene 3) are absent. Further results show that concatemers cannot be maintained in the absence of the exonuclease unless the endonuclease is also eliminated. Therefore, concatemers are formed by a process other than normal phage recombination. Selective defects in the recombination system do interfere with the stability of concatemers, however.