Abstract

Introduction. B1-lymphocytes are a subpopulation of B-cells, the proportion of which in the spleen accounts for 5 % of the total number of B-cells. B1-lymphocytes predominantly secrete IgM, which plays an important role in the induction of tumor cell apoptosis. Splenec tomy for the purpose of adequate lymph node dissection in gastric cancer causes marked and prolonged immunological disorders. It primarily affects the B1a-lymphocyte subpopulation, which provides a thymus-independent immune response. Objective. To study the features of the B-cell component of immunity in patients with gastric cancer. Materials and methods. The subpopulation composition of B-lymphocytes of peripheral blood of gastric cancer patients undergoing sur gical treatment using flow cytometry (Facs Can, Lysys II and FacsCanto II programs, Facs Diva program) was analyzed. Cells were stained simultaneously with three monoclonal antibodies labeled with different fluorochromes. Results. The data obtained demonstrate a violation of the composition of B-cell subpopulations. In the group of patients with standard D2 lymph node dissection and splenectomy at the preoperative stage and three months after surgery, a significant correlation was found between the relative number of B lymphocytes (p = 0.018), CD5 + B lymphocytes (p = 0.012) and the number of CD19 + CD38 + cells (p = 0.035). In addition, after surgical treatment, the percentage of CD5 + B-lymphocytes increased significantly from 12.9 to 21.8 %, while the total number of CD19 + lymphocytes and CD19 + CD21 + cells decreased. Conclusion . In patients of the experimental group, a decrease in antibody production, weakening of both general and antitumor immunity can be observed.

Highlights

  • B1-lymphocytes are a subpopulation of B-cells, the proportion of which in the spleen accounts for 5 % of the total number of B-cells

  • B1-lymphocytes predominantly secrete IgM, which plays an important role in the induction of tumor cell apoptosis

  • In the group of patients with standard D2 lymph node dissection and splenectomy at the preoperative stage and three months after surgery, a significant correlation was found between the relative number of B lymphocytes (p = 0.018), CD5 + B lymphocytes (p = 0.012) and the number of CD19 + CD38 + cells (p = 0.035)

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Summary

Оригинальные статьи

В1-лимфоциты секретируют преимущественно IgM, который играет важную роль в индукции апоптоза опухолевых клеток. Цель исследования – изучить особенности В-клеточного звена иммунитета у больных раком желудка. Проанализирован субпопуляционный состав В-лимфоцитов периферической крови больных раком желудка, подвергшихся хирургическому лечению методом проточной цитометрии (Facs Can, программа Lysys II и FacsCanto II, программа Facs Diva). В группе больных со стандартной D2-лимфодиссекцией и спленэктомией на дооперационном этапе и спустя 3 мес после операции выявлена достоверная корреляция между относительным количеством В-лимфоцитов (p = 0,018), CD5+В-лимфоцитов (p = 0,012) и количеством CD19+CD38+-клеток (p = 0,035). После хирургического лечения значительно возрос процент CD5+В-лимфоцитов – с 12,9 до 21,8 %, тогда как суммарное количество CD19+ лимфоцитов и CD19+СD21+ клеток снижалось. У больных опытной группы может наблюдаться снижение антителoпродукции, ослабление как общего, так и противоопухолевого иммунитета. Ключевые слова: B-лимфоциты, тимуснезависимые антигены, гуморальный иммунитет, рак желудка, спленэктомия. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia; 24 Kashirskoe Shosse, Moscow 115478, Russia

Introduction
Conclusion
Оригинальное имя Экспрессия на клетках
PE FITC
Findings
До операции
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