Abstract
BackgroundAcetyl-CoA carboxylase (ACC) plays an important role in the metabolism of various cancer cells, but its role in head and neck squamous cell carcinoma (HNSCC) is uncertain. Therefore, in the present study, we explored the role of ACC2 in HNSCC.MethodsWestern blot and immunohistochemistry assays were used to determine ACC2 protein expression levels in laryngocarcinoma and adjacent normal tissues derived from patients with laryngocarcinoma. ACC2 expression was knocked down in the hypopharyngeal cancer cell line FaDu to determine its effect on apoptosis. Lipid oil red staining was used to test the change of intracellular lipid.ResultsThe results showed that the ACC2 protein was highly expressed in laryngocarcinoma and that the ACC2 expression level was positively associated with the clinical cancer stage and negatively associated with the degree of laryngocarcinoma cell differentiation. Kaplan–Meier analyses indicated that compared with patients having low levels of ACC2, those with high ACC2 levels had a decreased 5-year survival rate. The results of western blot and terminal deoxynucleotidyl transferase dUTP nick-end labeling assays showed that knockdown of ACC2 accelerated apoptosis in FaDu cells. Furthermore, knockdown of ACC2 significantly reduced the intracellular lipid levels in FaDu cells.ConclusionThese findings suggest that ACC2 may be an important prognostic marker for patients with HNSCC and that ACC2 may be a potential target in the treatment of HNSCC.
Highlights
62,000 new head and neck cancer diagnoses and more than 13,000 deaths associated with this cancer occur in the United States every year (Kabarriti et al, 2018)
Increased ACC2 expression in patients with laryngocarcinoma We demonstrated that the p-Acetyl-CoA carboxylase (ACC) expression level was significantly higher in laryngocarcinoma tissue than that in the adjacent tissue (Fig. S1), which was consistent with Su et al (2014) Findings
Knockdown of ACC2 decreases intracellular lipids levels and enhances FaDu cell apoptosis Because we demonstrated in the present study that ACC2 is highly expressed in laryngocarcinoma and the prognosis for patients with laryngocarcinoma is poor owing to its rapid metastasis and growth, we explored whether ACC2 affects apoptosis and proliferation in head and neck squamous cell carcinoma (HNSCC)
Summary
62,000 new head and neck cancer diagnoses and more than 13,000 deaths associated with this cancer occur in the United States every year (Kabarriti et al, 2018). Treatment of head and neck squamous cell carcinoma (HNSCC) has greatly. The role of acetyl-coA carboxylase in head and neck squamous cell carcinoma. Several biomarkers of HNSCC, including SDF-1, CXCR4, HMGA2, CDKN2A, and TRPP2 (De-Colle et al, 2017; Palumbo et al, 2018; Chen et al, 2018; Wu et al, 2016), may potentially be used as therapeutic targets to overcome HNSCC. Acetyl-CoA carboxylase (ACC) plays an important role in the metabolism of various cancer cells, but its role in head and neck squamous cell carcinoma (HNSCC) is uncertain. Methods: Western blot and immunohistochemistry assays were used to determine ACC2 protein expression levels in laryngocarcinoma and adjacent normal tissues derived from patients with laryngocarcinoma. Conclusion: These findings suggest that ACC2 may be an important prognostic marker for patients with HNSCC and that ACC2 may be a potential target in the treatment of HNSCC
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