Abstract
The D1S80 locus is very useful for personal identification in Japan. To analyze PCR amplification products at the D1S80 locus, DIG-labeled primer was used for PCR amplifications. After electrophoresis, the PCR products were transferred to a nylon membrane and detected with alkaline phosphatase-labeled anti-DIG antibody (AP-DIG Ab). Numerous extra bands were detected on the membranes, indicating that PCR amplification products at the D1S80 locus contain many extra products which cause the undesirable bands to appear during D1S80 typing. To obtain a correct genotype, it was necessary to perform Southern blotting using an oligonucleotide that includes an internal sequence of the amplification products as a probe.
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