Abstract

. 1996). The large size of ITS1 makes directsequencing of this region difficult, so restriction fragmentlength variation analysis of PCR-amplified fragments(PCR-RFLP) is an alternative strategy. One may also focuson ITS2, which is suitable for direct sequencing, or alter-native DNA screening techniques such as single-strandconformation polymorphism analysis of PCR-amplifiedfragments (PCR-SSCP, Sheffield

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