The Retina-Attached SCN Slice Preparation: An In Vitro Mammalian Circadian Visual System

Abstract

The suprachiasmatic nucleus (SCN), the mammalian circadian pacemaker, receives information about ambient light levels through the retinohypothalamic tract. This information resets the molecular clock of SCN neurons, thereby entraining overt animal behavior and physiology to the solar cycle. Progress toward functional characterization of retinal influences on the SCN has been hampered by limitations of established experimental paradigms. To overcome this hurdle, the authors have developed a novel in vitro preparation of the rat retinohypothalamic circuit that maintains functional connectivity between the retinas and the SCN. This method permits whole-cell patch-clamp recordings from visually identified, light-responsive SCN neurons. Using this preparation, the authors have found that in the SCN, light-evoked responses are partly driven by the melanopsin photosensory system of the intrinsically photosensitive retinal ganglion cells and that SCN neurons exhibit light adaptation. The authors have also been able to generate this preparation from mice, demonstrating the feasibility of applying this method to transgenic mice.