The results of experimental-clinical study of healing of burn wounds and prevention of their complications under the influence of nano-containing biodegradable polymer technologies

Abstract

Burns cause high rates of morbidity and mortality worldwide. Improper initial treatment or delayed initial treatment can have adverse consequences for further wound healing. Goal. To study the effect of biodegradable polymer films «biodep-nano» on the healing of burns of the skin and prevention of complications. The aim of the study was to investigate the effect of our developed biodegradable polymer film «biodep-nano», which is additionally saturated with hydrated fullerene C60 on the healing of severe suppurative burns in animal experiments and the effectiveness of this film in the prevention of secondary infection in patients with mild burns. Material and methods. Simulation of skin burns was performed on 50 adult white Wistar rats weighing an average of 300.0 g, St.Aureus was injected under the scab, after 3 days the scab was removed surgically and the wound was cleaned with a biodep-nano film, the material was replaced every 2 days. with planimetric, microbiological and pathomorphological examination on the 3rd, 7th, 14th and 21st day. In the conditions of the burn department of Ivano-Frankivsk OKL, the biodegradable polymer film “biodep nano” introduced into production was used to prevent secondary infection of burn wounds. Results. When using the film developed by us, the area decreased by 21.6 % on the 3rd day, by 44.5 % on the 7th day, by 67.8 % on the 14th day. By 21 days there was a complete closure of the wound defect. The results of the microbiological study indicate that the level of contamination of burn wounds after application of the studied film of zinc nanooxide decreased during treatment and on the 3rd day to (4.3±3.3)*105 CFU/ml, which is the level of bacteria that is not pathogenic. After the 14th day, the pathogen was not sown and the wounds healed well. The obtained data showed that in group I where the film was used, the secondary infection was 11 % lower, which in turn showed a shorter wound healing time, which was 2 days less than in group II. Conclusions. The biodegradable material developed by us reliably protects burn wounds from accidental infection, is safetyless, elastic, and in experimental research demonstrated high medical properties in relation to the purulent burn wounds of IIIb century.