Abstract
The paper presents results of the production and use of the phytofortificant of cytoplasmic sperm membranes based on legume hydrolysate with additional purification with sorbents for cryopreservation of bull sperm as an alternative to yolk diluents. The aim of the research was to achieve biosafety, that is, to avoid infectious gynecological diseases, agents of which can be transmitted by the yolk, to increase the fertility rate of females after artificial insemination and to create the conditions for the development of new long-term cryoprotective diluents. It has been established that legumes grain and their hydrolysates contain a toxic nickel element that adversely affects the cytoplasmic membrane of sperm. To reduce this influence, we conducted a study of the action of different sorbents. Activated charcoal standard, silicon dioxide and fine silica were used as sorbents. Sorbents were added in relation of 3% of the volume of medium. According to the results of the studies, the best results were in semen, diluted with medium using high-dispersion silicon dioxide
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