The restriction analysis of nDNA from the corvids (Passeriformes, aves)

Abstract

1. 1. The nDNA of carrion crow Corvus corone L., hooded crow C. cornix L., their hybrids, as well as magpie Pica pica L., were digested by the tetranucleotide recognizing restriction enzymes Sau3a, AluI, BspRI and then analysed using electrophoresis with microdensitometry. 2. 2. The distribution patterns of restriction DNA fragments proved to be nuclease- and taxon specific. 3. 3. The observed families of repeated sequences are characterized by different length (from 30 bp to 23 tbp), number of copies in genome (approximately 10 3 and 10 6) and supposedly different types of organization and evolutionary age. 4. 4. The total DNA amount identified in the form of discrete fragments is 16 and 19–21% for magpie and crows, respectively. 5. 5. The DNA restriction patterns of hybrid forms do not differ from the parental species.