The responsiveness of Triticum aestivum L. variety for inoculation by cells of endophytic strains Bacillus subtilis.

Abstract

In this study, we tested the effect of two strains of bacteria B. subtilis 26D and 11ВМ on three varieties of wheat Triticum aestivum L.: Omskaya 35, Kazakhstanskaya 10 (spring), Volzhskaya qualitative (winter).The peculiarity of the plants response to endophytic inoculation depended on the strain of the microorganism, the concentration of cells in the preparation, and the variety of wheat during the experiment in Petri dishes. Both strains showed a strong growth-stimulating effect when seed was inoculated with suspensions of bacteria with a concentration of 106 cells/ml. There was no effect when seed cells were inoculated with bacteria at a concentration of 109 cells / ml. Plants varieties Omskaya 35 were most responsive to inoculation with endophytes. The variety was well responsive to the inoculation of bacteria cells at different concentrations. The variety Volzhskaya quality had the least growth stimulation. Plants of this variety responded well when grown in soil, unlike experiments in Petri dishes. The variety Kazakhstanskaya 10 was less responsive when growing plants in Petri dishes. There was no difference between the size of the shoots of inoculated and non-inoculated plants of the variety Kazakh 10, only stimulation of root growth was observed. It was concluded that there is a pronounced responsiveness of wheat varieties to the effect of endophytic strains of bacteria B. subtilis 26D the basis of biofungicide (Fitosporin-M) and this must be considered when using biofungicide for wheat cultivation.