Abstract

In barley aleurone layers, gibberellic acid (GA3) induces the synthesis of α-amylase while abscicic acid (ABA) inhibits this synthesis. The level of translatable mRNA for α-amylase is increased by GA3 and by GA3+ABA but it is not known if this increase is due to a direct enhancement of transcription of α-amylase gene by GA3 (transcriptional control) or to the processing of a precursor RNA (post-transcriptional control). To answer this question we have measured the levels of α-amylase specific mRNA sequences in barley aleurone layers using [32P]DNA complementary to α-amylase mRNA. The results show that exposure of barley aleurone layers to GA3 or GA3+ABA, leads to an increased accumulation of mature (∼ 1.6 Kb) α-amylase mRNA sequences.

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