Abstract
Brassinosteroids (BR) plays an important role in regulating plant growth. We found that the expression of BR synthesis and signal transduction genes were up-regulation when potato dormancy was released, and suckering agents can inhibit the expression of Methylsterol monooxygenase 1 (SMO1), Delta24-sterol reductase 1 (DWF1), Protein BR insensitive 1 (BRI1), BR-signaling kinase (BSK) and Cyclin D3 (CYCD3) genes in potato tuber. Besides, we found that the storage period among different cultivars are significant different at low temperature. Low temperature can obviously extend cultivar ‘Favorita’ storage time than cultivar ‘Mira’. In order to explore the response of BR genes to potato sprout at low temperature. This study will analyze the expression of BR synthesis, signal transduction and regulation genes at from cultivar ‘Favorita’ and cultivar ‘Mira’ which both cultivars are storage at low temperature (4°C) and room temperature (23±2)°C environment by qRT-PCR technique. The results showed that the expression of DWF1 and BRI1 are decreased in both cultivars by low temperature treatment. The expression of SMO1 , BSK and CYCD3 genes are decreased in cultivar ‘Favorita’by low temperature treatment. While, the expression of SMO1 , BSK and CYCD3 genes in‘Mira’are increase by temperature treatment. It shows that low temperature influences the expression of SMO1 , BSK and CYCD3 genes in cultivar ‘Favorita’, which results in the long dormancy period of the cultivar ‘Favorita’. These results will provide the theoretical basis in elucidating the BR mechanism of potato sprout, breeding variety selection and new technology research and development of potato storage regulation.
Highlights
As the fourth largest grain crop in China, potato is a both grain and vegetable crop, which is rich in amino acids, vitamins, mineral elements, etc. (Tian et al, 2017)
We found that the expression of BR synthesis and signal transduction genes were up-regulation when potato dormancy was released, and suckering agents can inhibit the expression of Methylsterol monooxygenase 1 (SMO1), Delta24-sterol reductase 1 (DWF1), Protein BR insensitive 1 (BRI1), BR-signaling kinase (BSK) and Cyclin D3 (CYCD3) genes in potato tuber
1.1 Sprout length measurement of ‘Favorita’ and ‘Mira’ Observed the microscopy of potato apical sprout in ‘Favorita’ at room temperature and low temperature (Figure 1), we found that at room temperature the apical sprout length was less than 1 mm at the time of storage of 0~19 d, on the 20th day, the apical sprout was gradually extended, and on the 37th day, it was more than 2 mm, the dormancy was released
Summary
As the fourth largest grain crop in China, potato is a both grain and vegetable crop, which is rich in amino acids, vitamins, mineral elements, etc. (Tian et al, 2017). Potato storage plays an important role in potato industry. About 4.1 million tons of potatoes are lost each year in China because of improper storage (Zhang et al, 2012). The dormancy period of potato is affected by temperature, humidity, and growth regulator. Low temperature is often used as the main method to prolong the storage time of potato (Si et al, 2007, Chinese Potato Journal, (2): 104-107). The dormancy period of potato is affected by external conditions, and closely related to its own genotype (Bamberg, 2010; Fan et al, 2009). We found that brassinosteroids (BR) plays a key role in dormancy release and germination of potato
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