Abstract
The resolution of a mixed system of alkyl ethers and monoglycerides, as their trimethylsilyl ether derivatives, has been studied by gas—liquid chromatography on several different types of liquid phase. Individual monoglyceride and alkyl ether derivatives up to C18:o were resolved on non-polar columns in under 25 minutes and best separations were achieved on JXR methyl silicone liquid phase. Semi-polar and polar columns did not separate saturated and mono-unsaturated TMS derivatives of either alkyl ethers or monoglycerides. Contamination detected in alkyl ether samples prepared by acid hydrolysis of the glycerogalactolipid fraction of sheep brain has been identified as monoglyceride.
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