The resistance of upland rice doubled haploid lines to blast diseases and brown planthopper

Abstract

The objective of this study was to determine the resistance of doubled haploid lines of upland rice to leaf blast race 033, 073, 133, 173 and brown planthopper biotype 1, 2 and 3. The experiments were conducted in the Muara greenhouse, Indonesia Center for Rice Research (ICRR), Bogor-West Java Province. All experiments were conducted in the span of October 2017 to April 2018. The materials used in the research were 14 upland rice doubled haploid lines, 2 check varieties namely Inpago 10 and Limboto. Testing the resistance of doubled haploid rice of upland rice to blast disease was coupled with differential varieties i.e Asahan as blast-resistant check variety and Kencana Bali as blast susceptible check variety, while to brown planthopper with differential varieties i.e TN1, Pelita, IR26, IR42, PTB 33 and Rathu Heenati. The experimental design was a randomized complete block design with three replications. Resistance study of doubled haploid lines to leaf blast showed that ST7 and Limboto were resistant to race 033 while ST4, ST7, ST9, ST10, ST12, and Limboto were resistant to race 073. Meanwhile, ST4, ST9, ST10, ST11, ST12, Limboto and Inpago 10 were resistant to race 173. Four doubled haploid lines were moderately resistant to race 133 i.e. ST6, ST12, ST4, and ST14. The highest disease intensity was found in race 133 (52.2%) followed by race 033 (35.9%), race 173 (30.4%) and the lowest in race 073 (28.1%). Based on IRRI method of evaluation, two doubled haploid lines of upland rice i.e. ST1 and ST3 were moderately resistant to the biotypes of brown planthopper.