Abstract
The present study aims to investigate the response of rapeseed microspore-derived embryos (MDE) to osmotic stress at the proteome level. The PEG-induced osmotic stress was studied in the cotyledonary stage of MDE of two genotypes: Cadeli (D) and Viking (V), previously reported to exhibit contrasting leaf proteome responses under drought. Two-dimensional difference gel electrophoresis (2D-DIGE) revealed 156 representative protein spots that have been selected for MALDI-TOF/TOF analysis. Sixty-three proteins have been successfully identified and divided into eight functional groups. Data are available via ProteomeXchange with identifier PXD024552. Eight selected protein accumulation trends were compared with real-time quantitative PCR (RT-qPCR). Biomass accumulation in treated D was significantly higher (3-fold) than in V, which indicates D is resistant to osmotic stress. Cultivar D displayed resistance strategy by the accumulation of proteins in energy metabolism, redox homeostasis, protein destination, and signaling functional groups, high ABA, and active cytokinins (CKs) contents. In contrast, the V protein profile displayed high requirements of energy and nutrients with a significant number of stress-related proteins and cell structure changes accompanied by quick downregulation of active CKs, as well as salicylic and jasmonic acids. Genes that were suitable for gene-targeting showed significantly higher expression in treated samples and were identified as phospholipase D alpha, peroxiredoxin antioxidant, and lactoylglutathione lyase. The MDE proteome profile has been compared with the leaf proteome evaluated in our previous study. Different mechanisms to cope with osmotic stress were revealed between the genotypes studied. This proteomic study is the first step to validate MDE as a suitable model for follow-up research on the characterization of new crossings and can be used for preselection of resistant genotypes.
Highlights
Plant breeding is focused on the permanent increase of crop production to meet the needs of an ever-growing world population, improving food quality to ensure long and healthy life, and to address the problems of global warming and environmental pollution, together with the challenges associated with developing novel biofuel sources
Two cultivars of winter oilseed rape differing in their drought-adaptation strategies were included in this analysis
Spots found in this study show that the antioxidant system and ROS production play a crucial role in microspore-derived embryos (MDE) resistance and should be further examined to contribute to the selection of adaptable rapeseeds
Summary
Plant breeding is focused on the permanent increase of crop production to meet the needs of an ever-growing world population, improving food quality to ensure long and healthy life, and to address the problems of global warming and environmental pollution, together with the challenges associated with developing novel biofuel sources. A combination of different approaches (mechanistic physiological understanding, -omics, association mapping, envirotyping, gene editing, and other tools) need to be utilized to improve significantly the abiotic stress resistance of crops in the field (Mittler and Blumwald, 2010). Despite the importance of oilseed rape worldwide, between the years 2005 and 2021, only four drought-focused comparative proteomic studies were aimed at B. napus [seedling roots (Mohammadi et al, 2012), 20-day-old seedlings (Koh et al, 2015), and mature plants (Wang et al, 2016; Urban et al, 2017)]. NaCl and PEG solution proteomic analysis on 15day-old seedlings of one cultivar was studied by Luo et al (2015)
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