Abstract
In 2006, 54 pasteurized full fat milk samples, 40 ice-cream samples, and two green-tea beverage samples were analyzed and a total of 19 Bacillus thuringiensis-like strains were isolated, nine from seven pasteurized milks, one from an ice-cream with peach pulp and juice, and nine from two green-tea beverages. These strains were classified as B. thuringiensis, contained the cry1A gene and produced crystal inclusions during sporulation. All strains were characterized by a serotyping test, SDS-PAGE, random amplified polymorphic DNA, and enterotoxic gene PCR analysis. Most isolates produced bipyramidal crystals and belonged to serotypes H 3a3b, H 5a5b, or H 7. Furthermore, two strains from pasteurized full fat milks and three strains from green-tea beverages were indistinguishable from the B. thuringiensis subsp. kurstaki strains isolated from commercial biopesticides (Kaiyan®, Qiangdi®, Lvpuan® and Sutai®), suggesting the residual occurrences of B. thuringiensis from biopesticides in food and beverages.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
