Abstract
Objective To detect the enrichment of breast cancer stem cells induced by transforming growth factor β1 (TGF-β1),and the difference of p21 expression between breast cancer stem cells and MCF7/ADM cells.Methods Flow cytometry detect the proportion of aldehyde dehydrogenase Ⅰ (ALDH1) positive cells in MCF7/ADM,breast cancer stem cells enriched by mammosphere and breast cancer stem cells induced by TGF-β1.To detect the mammoshpore formation ability in above three teams by mammoshpere formation test.To detect the p21 expression in three teams above by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting.Results The proportion of ALDH1 positive cells in the three teams were 8.49%,14.94%,23.44% respectively.The mammosphere formation rate were 5.29%,11.67%,23.13% respectively.The expression of p21 increased in the three teams.Conclusion TGF-β1 can induce the enrichment of breast cancer stem cells,and enhance it' s self-renewal ability.The expression of p21 is higher in breast cancer stem cells than common breast cancer cells. Key words: Breast cancer stem cell; Tranforming growth factor-β1 ; Aldehyde dehydrogenase 1 ; p21
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