Abstract
Objective To detect the enrichment of breast cancer stem cells induced by transforming growth factor β1 (TGF-β1),and the difference of p21 expression between breast cancer stem cells and MCF7/ADM cells.Methods Flow cytometry detect the proportion of aldehyde dehydrogenase Ⅰ (ALDH1) positive cells in MCF7/ADM,breast cancer stem cells enriched by mammosphere and breast cancer stem cells induced by TGF-β1.To detect the mammoshpore formation ability in above three teams by mammoshpere formation test.To detect the p21 expression in three teams above by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting.Results The proportion of ALDH1 positive cells in the three teams were 8.49％,14.94％,23.44％ respectively.The mammosphere formation rate were 5.29％,11.67％,23.13％ respectively.The expression of p21 increased in the three teams.Conclusion TGF-β1 can induce the enrichment of breast cancer stem cells,and enhance it' s self-renewal ability.The expression of p21 is higher in breast cancer stem cells than common breast cancer cells. Key words: Breast cancer stem cell; Tranforming growth factor-β1 ; Aldehyde dehydrogenase 1 ; p21
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