Abstract
Recently, the share of medicinal products of natural origin has increased in the pharmaceutical market of Ukraine. This is due to the fact that the pharmacological effect of natural substances provides a complex of biologically active substances, which has a high therapeutic effect and the minimum number of adverse reactions. Therefore, the study of chemical composition and standardization of natural substances is an important stage in pharmaceutical research.A promising source of active ingredients is the mushroom Pleurotus ostreatus. The chemical composition of this fungus is represented by a large number of biologically active substances, which ensures its many-sided use in folk medicine.Aim. The determination of qualitative and quantitative composition of free amino acids in the composition of water-soluble protein-polysaccharide complex (WPPSC) of oyster mushroom Pleurotus ostreatus was the aim of research.Methods. Physicochemical methods are the most commonly used to study active substances of natural origin. This is due to the fact that they provide high informative, accurate, efficient and reproducible. The method of high performance liquid chromatography (HPLC) was used to analyze the amino acid composition of the WPPSC of oyster mushroom Pleurotus ostreatus. This method is based on the separation of individual components due to different adsorption capacity.Results. Research of the amino acid composition of the WPPSC of oyster mushroom Pleurotus ostreatus showed that the complex contains 10 (7 %) free amino acids, among which 5 are essential. The detected aminoacids are aliphatic, heterocyclic and aromatic compounds. They are presented by glycine, alanine, leucine, isoleucine, threonine, selinum, lysine, arginine, histidine and phenylalanine.Conclusion. The qualitative and quantitative composition of the free amino acids of the WPPSC of oyster mushroom Pleurotus ostreatus was established. The method of determination and separation of free amino acids by the method of high-performance liquid chromatography is proposed. The obtained results are proposed for standardization of the initial substance in the process of production of medicines based on WPPSC of oyster mushroom Pleurotus ostreatus
Highlights
Natural herbal medicines are very popular with people around the world
An important stage in the production of medicines is the development of methods for standardizing biologically active substances of natural origin that will be able to provide the predicted therapeutic effect of medicines created on their basis [2]
The field of research considering the general problem, which is described in the article To date, the unidentified composition of the amino acid part of the water-soluble protein-polysaccharide complex (WPPSC) of the Pleurotus ostreatus fungus
Summary
Natural herbal medicines are very popular with people around the world. This is due to the fact that complexes of biologically active substances of natural origin provide high efficiency and insignificant number of side effects. Natural active substances are multicomponent mixtures, the qualitative and quantitative composition of which can vary in the process of ontogenesis of the plant organism, and as a result of the technological process of obtaining, processing and storing raw materials, isolated from it substances and medicines created on their basis.
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