The research of copper enrichment in four types of shellfish

Abstract

The copper enrichment effects in four types of shellfish including Tegillarca granosa,Ruditapes philippinarum,Sinonovacula constricat canarck and Monodonta labio were studied in this paper.The study was carried out through the static test method.The shellfish was cultivated in seawater contaminated with different copper concentrations(0.015,0.02,0.025,0.035,0.065 and 0.115 mg/L,respectively).The content of Cu was analyzed in muscle tissue using atomic absorption spectrometry with microwave digestion in 0,1,3,5,10,15,20,25 and 30 d,respectively.The results indicated that the four types of shellfish had copper enrichment effect.The concentration of copper in monoplacophora was higher than that in bivalvia.The copper content in T.granosa and M.labio exhibited significant increases while the concentration of copper in seawater was higher than 0.035 mg/L;The copper content in R.philippinarum and S.constricat canarck exhibited significant increases while the concentration of copper in seawater was higher than 0.025 mg/L.As a whole,the levels of copper in shellfish increased with the increase of that in seawater.When the concentration of copper in seawater increased to a value,the copper content in shellfish was low.The value was 0.025 mg/L for T.granosa and 0.035 mg/L for R.philippinarum,M.labio.As a whole,the levels of copper in shellfish increased with the increase of enrichment time.The levels of copper in shellfish showed significant decrease in 3 5 d and continued to increase after 5 10 d.The mechanisms of shellfish response to copper enrichment were investigated in this paper.