Abstract
It is possible to prepare ribosomes from Escherichia coli which are virtually free from contamination by GTP, supernatant proteins, and active sRNA. Such preparations have been employed to determine the minimum requirements for the binding of a specific sRNA by the ribosomes. These are an appropriate mRNA to specify which sRNA shall be bound and ionic conditions that will permit the formation of a stable complex. There is no requirement for supernatant factors or GTP. The sRNA can be bound equally well in the amino-acylated form or in the deacylated form. It is concluded that the structural properties of the sRNA, mRNA and the ribosomes are sufficient per se to determine the specificity of their interaction.
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