Abstract
An Aedes triseriatus cell line was established from neonatal larval tissue. In the 60th passage, the cells have been characterized as to growth, chromosome number, absence of microbial contamination, and absence of La Crosse encephalitis virus (LAC) and other vertebrate viruses as determined by passage in suckling mice and electron microscopy. Isozyme analysis using polyacrylamide gel electrophoresis of lysed cell suspensions and suspensions of Aedes triseriatus larvae revealed that 4 enzyme phenotypes (isocitrate dehydrogenase, malic enzyme, phosphoglucoisomerase, and phosphoglucomutase) were identical. The Aedes triseriatus cells grow in plastic Falcon flasks in a medium (MM/VP12) composed of equal volumes of Mitsuhashi-Maramorosch and Varma-Pudney media with fetal bovine serum and antibiotics. The growth characteristics of 2 California serogroup viruses (La Crosse and trivittatus) in these cells are described
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