Abstract
Nymphs and adults of H. rufipes were individually inoculated intracoelomically with 0.02 ml of Congo virus having a titre of 3.5. LD 50/0.02 ml. The virus replicated in both nymphs and adults. Peak virus contents of 7.4 LD 50/0.02 ml and 4.6 LD 50/0.02 ml were obtained in inoculated nymphs and adults, respectively. Congo virus infection, acquired in the nymphal stage, persisted during metamorphosis and was passed on to the adult. Engorged adults had a significantly higher virus content than unfed adults. Unfed male and female adults had about the same amount of virus until engorgement, when the level of virus in the engorged gravid female rose higher than that in males. Virus was detected in the unfed adults, 185 days post inoculation.
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