Abstract
In previous studies, the abundance and diversity of methanogenic archaea in the dental microbiota have been analysed by the detection of specific DNA sequences by PCR-based investigations and metagenomic studies. Few data issued regarding methanogens actually living in dental plaque. We collected dental plaque specimens in 15 control individuals and 65 periodontitis patients. Dental plaque specimens were cultured in an anoxic liquid medium for methanogens in the presence of negative control tubes. Dental plaque methanogens were cultured from 1/15 (6.67%) control and 36/65 (55.38%) periodontitis patient samples (p<0.001). The cultures yielded Methanobrevibacter oralis in one control and thirty-one patients, Methanobrevibacter smithii in two patients and a potential new species named Methanobrevibacter sp. strain N13 in three patients with severe periodontitis. Our observations of living methanogens, strengthen previous observations made on DNA-based studies regarding the role of methanogens, in periodontitis.
Highlights
In previous studies, the abundance and diversity of methanogenic archaea in the dental microbiota have been analysed by the detection of specific DNA sequences by PCR-based investigations and metagenomic studies [1]
These analyses have revealed that the dominant methanogenic archaea in the oral cavity was Methanobrevibacter oralis [1,2,3,4,5,6]
Realtime PCR was negative in tubes that did not produce methane and positive in the methaneproducing tube in the control group and in 31/36 (86.11%) methane-producing tubes in the patient group (p
Summary
The abundance and diversity of methanogenic archaea in the dental microbiota have been analysed by the detection of specific DNA sequences by PCR-based investigations and metagenomic studies [1]. These analyses have revealed that the dominant methanogenic archaea in the oral cavity was Methanobrevibacter oralis [1,2,3,4,5,6]. The sequences indicative of original phylotypes corresponding to yet uncultured methanogens have been found [6,8,9,10] Such molecular approaches do not PLOS ONE | DOI:10.1371/journal.pone.0121565. Such molecular approaches do not PLOS ONE | DOI:10.1371/journal.pone.0121565 April 1, 2015
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
