Abstract

Several methods are presently used to identify and quantify fumonisins in foods and feeds. HPLC procedures on derivatized fumonisins with fluorescence detection are most commonly used. The validity and significance of reported fumonisin levels depend on several factors such as the specificity, detection limit, accuracy and reproducibility of the analytical method as well as on the sampling procedure used, and the integrity and purity of the analytical standards. The importance of these factors is discussed and the results of two international collaborative studies are presented on the determination of fumonisins in corn by a reversed-phase HPLC method on o-phthaldialdehyde (OPA) derivatized fumonisins using fluorescence detection.

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