Abstract
THE OBJRCT of this investigation was to male a quantitative study of the estrogens found in hen-s ovaries, immature ova, follicular membranes, and in fresh-laid yolks.of this and his coworkers investigation1 extractedwas to make a quantitativethe follicles from hen ovariesstudy withof the estrogens alcohol and were able to show that their extract contained an estrogenic hormone from its effect on the uteri of spayed rats. Their study of fresh egg yolk gave a negative response. They used, however, only 18 yolks. Glimm and Wadehn2 were unable to detect the estrogenic character, using 10 egg yolks and noting the growth of the uteri of rabbits. Serone and Montemolo3 showed that the estrogenic substance was evident in egg yolk only upon progressive incubation of the egg. Fellner4 reported that the lipoid fraction of egg gave a positive test for estrogens when rabbits were used. Kopec and Greenwood5 confirmed the work of Fellner by injecting 5 egg yolks into a poulard over a period of 20 days.
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