Abstract
There are eight most abundant green tea catechins (GTCs) existing in four pairs of eipimers, and carbon-3 configuration represents the only steric difference within each pair. This study aimed to use a new kinetic approach to elucidate the effect of stereochemical changes on the antioxidant activity. A mixture of eight GTCs was treated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) prepared in a series of concentrations, their relative reaction rates towards scavenging DPPH were revealed by the recently introduced parameter, i.e. Dm. The 3-R configuration in (−)-epicatechin, (−)-epigallocatechin and (−)-epigallocatechin gallate gave lower Dm values demonstrating faster kinetics as opposed to their 3-S counterparts, with the only exception of the pair of (−)-catechin gallate and (−)-epicatechin gallate in which 3-S configuration was faster. These results suggested that the kinetic approach adopted in this study could reflect the different antioxidant activity of GTCs attributed by minor steric changes.
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