The relationships between changes in main biochemical parameters in sperm cells of infertile men

Abstract

Detection of the regularities of functions of male genital cells, playing a principal role in the reproductive realization (environmental and psychological stresses) is especially relevant. Thus, the definition of the biochemical parameters of sperm at patho­spermia is an actual problem of medical biochemistry and laboratory diagnostic. A cluster analysis was performed to determine the distribution of men (normozoospermic and with different semen abnormalities – oligo-, astheno-, oligoasthenzoospermia and leukocytospermia) by groups according to biochemical indices. To determine a degree of affinity between the individual biochemical indicators and distinguish the most important changes, the obtained results were analyzed by principal component analysis (PCA). Based on parameters of glutathion antioxidant system (GSG, GP, GR, GST), arginase/NO-synthase system (cNOS, iNOS, arginase), membrane-bound ion transporting enzymes (Na + ,K + - and Ca 2+ , Mg 2+ -ATPase) and prooxidant manifestations (TBARS, GSSG), a cluster analysis of studied infertile male groups was performed. Normozoospermic males were the most distant from infertility men with leucocytospermia. However, the latter were involved in the formation of a subcluster which consisted of oligo-, astheno- and oligoasthenozoospermic groups. It is worth noting that oligo- and oligo­ashtenozoospermic males were the most related and formed a clear cluster based on the analyzed parameters. A usage of principal component analysis allowed quantitative pro­ving a degree of affinity between changes of the individual investigated parameters under the conditions of pathospermia. By means of principal component analysis of the studied parameters a consistency of changes in oxidative manifestation (TBARS and GSSG content), and the activity of glutathione-S-transferase and ion-transporting systems of spermal membranes of asthenozoospermic samples were set up. In the oligozoospermic samples the closest relations between the activity of the ion-transporting systems of the spermal membranes and the content of the lipoperoxidation products were detected.