Abstract
SYNOPSIS. As a part of an investigation of speciation in the Trypanosomatidae six species of crithidia were studied to determine their antigenic relationships. Antisera were prepared in chickens by means of three intravenous injections of several million crithidia and the titer and tests accomplished by using the agglutination technic. Titers against homologous protozoa averaged 1/640 with the highest titer recorded being 1/5120. Each antiserum was first tested against its homologous antigen then against each of the various crithidia. Results indicated the possibility that the crithidia isolated from hemiptera (C. acanthocephali, crithidia from Arilus cristatus and crithidia from Euryophthalmus davisi) were identical as far as our tests could determine. Those isolated from dipterons (C. fasciculata, C. luciliae) as well as C. (Strigomonas) oncopelti which was presumably isolated either from Oncopeltus fasciatus or the milkweed, Asclepias syriaca, were likewise identical. Each antiserum was adsorbed by each antigen and the serum tested against the homologous antigen. Again, with one possible exception, the close affinity between the dipteran flagellates (plus C. oncopelti) was established as was that between the flagellates of the hemipterons. Other than an occasional reaction, there were no cross reactions between the two groups of crithidia.
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