Abstract
Rat myotubes infected with polyoma virus (PV) introduced into the multinucleated cells by virus-bearing myoblasts at the time of cell fusion incorporate 3H-TdR and exhibit mitotic-type figures. The infected myotubes also produce a viral-specific nuclear antigen, tumor (T) antigen, which appears in groups of adjacent nuclei or in all nuclei of the myotubes. The proportion of myotubes which synthesize DNA, T-antigen and exhibit mitotic-type figures is related to the multiplicity of virus infection. Intact myotubes which are resistant to infection with PV by virus absorption can be infected by microinjection of the virus into the cells. Myotubes thus infected produce T-antigen which appears in multiple nuclei, but do not incorporate 3H-TdR or contain mitotic-type figures. The data suggest that the resistance of myotubes to infection with PV might be due to a change in the cell surface membrane during differentiation so that virus cannot penetrate the cell. The T-antigen apparently has no bearing on the activation of the DNA-synthesizing apparatus in multinucleated muscle cells.
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