Abstract
Nucleic acid (DNA) extraction using a Formalin-Fixed Paraffin-Embedded (FFPE) block is a frequent practice in pathological diagnosis and medical research. The extracted nucleic acid, which is the targeted end-product for the further molecular genetic analysis, depends on the ratio of tumor cells to normal cells. Theoretically, the same tissue sample would give an identical genetic detection result. However, the variable percentage of tumor cells and normal cells influences this theoretical result, therefore potentially influencing the final interpretation of this genetic testing result. To investigate the relationship between DNA isolation and biomarker detection, we studied biomarker detection using DNA isolated from FFPE samples and from secondary DNA isolation of leftover tissues that are usually available after the first round of lysis.
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