The Relationship of Hemoglobin Synthesis to Erythroid Colony and Burst Formation

Abstract

AbstractWe have demonstrated that the cyclohexaformation of large erythroid colonie none method for the extraction of hematin (bursts) resulting from the BFU-E (burstcan be used to measure hemoglobin forming unit, erythroid). The epo dosesynthesis induced by erythropoietin (epo) response curves for CFU-E colony counts in mouse bone marrow cells cultured in and day-2 hemoglobin synthesis were simimedium containing methyl cellulose. The lar, and the cell-number-response curves time course of hemoglobin synthesis by for these two parameters were parallel, mouse marrow cells showed two effects The epo dose-response curve for BFU-E due to epo: an increase in hemoglobin colony counts reached a plateau at an epo synthesis at day 2, which corresponded to concentration between 3 and 5 units /ml, the formation of small erythroid colonies whereas the dose-response curve for 6 resulting from the CFU-E (colony-forming 8-day hemoglobin synthesis did not reach a unit, erythroid), and a very large increase plateau even at an epo dose of 10 in hemoglobin synthesis, which was maxiunits/ml. mal at days 7-8 and corresponded to the formation of large erythroid colonies (bursts) resulting from the BFU-E (burst-forming unit. erythroid). The epo dose-response curves for CFU-E colony counts and day-2 hemoglobin synthesis were similar, and the cell-number-response curves for these two parameters were parallel. The epo dose-response curve for BFU-E colony counts reached a plateau at an epo concerntration between 3 and 5 units/ml, whereas the does-response curve for 6-8-day haemoglobin synthesis did not reach a plateau even at an epo dose of 10 units/ml.