The Relationship of BsmI Vitamin D Receptor Gene Polymorphism with Clinicopathological Picture of Breast Cancer Patients at Sanglah Hospital Denpasar Bali

Abstract

Introduction: Breast cancer is the most common cancer found in women and is one of the diseases associated with aging. Previos studies have found a significant association between the BsmI vitamin D receptor and breast cancer. This study aims to prove the relationship between BsmI vitamin D receptor polymorphisms with clinicopathological features of breast cancer at Sanglah Hospital, Denpasar Bali. Methods: This research is an analytic study with cross sectional method. The samples used in this study were 50 stored biological materials in the form of DNA from breast cancer patients in 2016- 2018 which is in the Biochemistry Laboratory of the Udayana Faculty of Medicine. To determine the polymorphism of the BsmI vitamin D receptor gene, DNA samples from breast cancer patients were amplified by polymerase chain reaction (PCR) method, then identified by sequencing method. The results obtained were then analyzed statistically using bivariate and multivariate analysis by using spss 25.sav. Results: 45 people (90.0%) were found with heterozygous polymorphism G/A and 5 people (10.0%) with homozygous wildtype GG. There was a statistically significant relationship between BsmI vitamin D receptor polymorphism and breast cancer stage at Sanglah Hospital Denpasar Bali (p=0.048). There was no significant relationship between age and history of contraceptive use and clinicopathology of breast cancer, but menstrual status was significant with the stage of breast cancer patients (p=0.037). Conclusion: There is a significant relationship between BsmI vitamin D receptor polymorphism and breast cancer stage at Sanglah Hospital Denpasar Bali.