Abstract
The onset and degree of aerobic fermentation of galactose during adaptation was found to be dependent upon the preliminary accumulation of reserves and intermediates and eventual saturation of the respiratory systems. Also, the oxidation of galactose resulted in the accumulation of fructose phosphates indicating that the oxidative breakdown of galactose also proceeds to some extent through the Embden-Meyerhof scheme. The accumulation of metabolic compounds in the cells increased at an even greater rate following the start of fermentation. An important implication of these results is that fermentation by whole cells is not a reliable criterion for measuring accurately the activity of the galactose-adaptive enzyme, and experiments using this method to demonstrate that adaptive enzyme synthesis is autocatalytic in nature are subject to error.
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