The Relationship Between the Colors and Anthocyanins of Clematis Hybridas with Double Petals

Abstract

In order to find out the relationship be tween the colors and anthocyanins of clematis hybridas ( Clematis florida Thunb. ) with double petals, 6 major anthocyanins in clematis hybridas with double petals of 4 color systems were quantitatively analyzed by HPLC-MS. The results showed that anthocyanins were detected in all clematis hybridas, but the monomer content and total amount of anthocyanins were significantly different, and the dominant anthocyanin monomer in different color systems were different. The contents of peonidin and cyanidin in red clematis hybridas were significantly higher than those in purple and blue clematis hybridas, up to 30.7 and 13.0 times, indicating that peonidin and cyanidin might be related to red coloring. The content of delphinidin in purple clematis hybridas was higher than that in red clematis hybridas, but lower than that in blue clematis hybridas, and the content of delphinidin in blue clematis hybridas was the highest, indicating that delphinidin content might affect the change of flower color from purple to blue. Only a little cyanidin was detected in white clematis hybridas, but no other five anthocyanins were detected, indicating that the pure white clematis hybridas should be free of anthocyanins, and the little cyanidin detected might be related to the reddish color at the end of the calyx petals of the test material. In addition, the total amount of 6 anthocyanins in red clematis hybridas was much higher than that in other color systems, indicating that the different colors of calyx petals was closely related to the total amount of anthocyanins. The implementation of this study has laid a foundation for exploring the coloration mechanism in calyx petals of clematis hybridas, and also provided a theoretical basis for the breeding of flower color of clematis hybridas.