Abstract
A 300 fold range of epididymal sperm concentrations (0.3–90 × 105/ml) has been tested to determine whether there is a relationship between sperm concentration and level of fertilization achieved in vitro of mouse eggs. While very low concentrations (0.3–1.25 × 105/ml) resulted in relatively low fertilization (43–;64 percent), those in the range of 2.5–;90 × 105/ml gave fertilization rates of 80–;94 percent. Consistently high results were obtained with sperm counts above 30 × 105/ml and there was no evidence of reduction in fertility with very high sperm concentrations. Likewise, no observable minimum was found; acceptable rates of fertilization were achieved with a mean sperm:egg ratio of 417:1.
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