The relationship between SNP rs895819 (A>G) on miRNA-27a and the breast cancer in the Vietnamese population

Abstract

Breast cancer (BC), the most common type of cancer among women worldwide, is a polygenetic disease which is caused by the interaction of several genes. Understanding the genetic factors for early diagnosis of BC is crucial to ensure the survival of BC patients. MicroRNA 27a (miR-27a), an oncogenic miRNA, has been predicted to target on the tumor suppressor ZBTB10 that can regulate many processes of cell. Single nucleotide polymorphism (SNP) rs895819 alters the structure and function of miR-27a, which has been anticipated to reduce the risk of BC in different populations such as German and Chinese. This study aimes to investigate the relationship between the existence of SNP rs895819 (A>G) and the risk of BC using the optimized high resolution melting (HRM) method. 106 BC samples and 117 healthy samples were genotyped using an optimal HRM protocol then statistical analysis was applied to examine the relationship of the SNP. In the case group, the risk G allele accounted for 36 % while in the control group it took up 32 %. Statistic result revealed that rs895819 (A>G) had no significant relationship with the breast cancer (OR=1.119; P=0.46676) in given case-control samples. Although the SNP is significantly ralated with BC in German or Chinese populations, it is not a potential marker for diagnosis in the Vietnamese population. Further studies investigating relationship between rs895819 (A>G) and breast cancer in the Vietnamese population is not recommended. In future, other SNPs should be investigated with the aim of identification efficient biomarker for early diagnosis of BC in Vietnamese.