The relationship between PRDM16 promoter methylation in abdominal subcutaneous and omental adipose tissue and obesity

Abstract

To explore the relationship between PR domain containing 16 (PRDM16) gene promoter methylation in abdominal subcutaneous adipose tissue (SAT) and omental adipose tissue (OAT) and obesity. Participants were divided into overweight/obesity (33 cases) and normal-weight groups (28 cases). PRDM16 promoter methylation in abdominal SAT and OAT were detected. Two independent sample t test, paired t test were used to analyze. (1) The methylation levels in overweight/obesity group and normal-weight group were different. There were 3 CpG sites different statistically between two groups. To chr1: 2987447 in SAT and chr1: 2986395 in OAT, the methylation level of normal-weight group was higher. While to chr1: 2987450 in OAT, the methylation level of normal-weight group was lower. (2) The methylation levels in SAT and OAT were different. There were 15 CpG sites different statistically in normal-weight group, and 22 CpG sites in overweight/obesity group. There were 7 CpG sites different statistically in both groups with their methylation levels being higher in OAT than that in SAT. (3) There were 9 haplotypes with statistical significance (8 in SAT, 1 in OAT). The methylation degree of PRDM16 gene promoter in abdominal SAT and OAT has the complex relationship with overweight/obesity. The variation of methylation haplotypes combination of multiple sites may play some roles.