Abstract
The absorption and fluorescence of cyanine, oxonol and merocyanine dyes are sensitive to changes in membrane potential [ 11. Although the optical response is usually complicated by contributions from cation binding [2,3], under carefully defined conditions the probes are reliable indicators of membrane potential in subcellular particles and in living cells. This report deals with the relationship between ion diffusion potential and the flux of calcium ions in sarcoplasmic reticulum vesicles isolated from rabbit skeletal muscle. The cyanine dye @i-O-C,(5)) was used as potential probe. Two questions were investigated. 1. Do potential changes accompany the ATP-mediated active uptake [4] and the (ADP + P&induced release of Ca2’ [5] by sarcoplasmic reticulum vesicles? 2. What is the influence of artificially-imposed membrane potential upon the rate of Ca2’ release from sarcoplasmic reticulum? Amherst College, Amherst, MA. Methanesulfonic acid and Di-0X2(5) were obtained from Eastman Kodak, Rochester, NY. Egg phosphatidylcholine was purchased from Sylvana Chemical Co., Millburn, NJ, chlortetracycline from Sigma Chemical Co., St Louis, MO, and arsenazo III from Aldrich Chemical Co ., Milwaukee, WI.
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